Study of the microbiota associated to Ruditapes decussatus and Ruditapes philippinarum clams by 16S rRNA metabarcoding, dilution to extinction, and culture-based techniques

Abstract The study of the microbiota associated to clams is important not only know their sanitary status but also prevent pathobiology events. use different microbiological techniques can help obtain a better picture bacterial diversity as well isolate new taxa. In this study, two clam species, Ruditapes decussatus and R. philippinarum , were analyzed in locations Galicia (northwest Spain) April October, by combining classic culturing, dilution-to-extinction approach, 16S rRNA gene target sequencing. sequencing revealed great within samples, shedding light into vast microbial communities these bivalves. All samples dominated same genera periods, namely Mycoplasma Vibrio, Cutibacterium . α-diversity obtained during month October was lower showed dominance rare taxa, such Methylobacterium or Psychrobacter Dilution-to-extinction technique demonstrated its usefulness culture taxa that found under culturing techniques, including Rahnella Brachybacterium, Micrococcus Jantinobacter, Lelliottia Altogether, our provides valuable information on philippinarum, demonstrating high complexity dynamics populations.